ABSTRACT
In this study we assessed the usefulness, healing, as well as the integration to lung tissue of glutaraldehyde preserved at 0.5 bovine pericardium GPBP and lyophilized (GPBPL), after reinforced resection of lung tissue in dogs by thoracotomy or thoracoscopy. MATERIAL AND METHODS: GPBP and GPBPL were prepared and used to reinforce the suture line of lung resection in 30 mongrel dogs: Group I (n = 6): The GPBP were fixed on the lung with 4-0 polypropylene by thoracotomy. Group II (n = 6): The resection and fixed of the GPBP were performed with an linear stapler by thoracotomy. Group III (n = 6): The resection and fixed of the GPBPL were performed with an linear stapler by thoracotomy. Group IV (n = 6): The resection and fixed of the GPBP strips were performed with a linear stapler by thoracoscopy. Group V: The resection and fixed of the GPBPL strips were performed with a linear stapler by thoracoscopy. Clinico-radiological evaluation was done until euthanasia of all animals at week 8 postop. Progressive insufflation up to 40 cm H2O of airway pressure was done to evaluated resistance of the heal in the suture line reinforced. Macroscopic, and microscopic examination of the GPBP, GPBPL and lung were evaluated. RESULTS: All animals survived the surgical procedure and study time (8 weeks). No airleaks were evident at any time during the study including the insufflation test. Macroscopic examination of the GPBP and GPBPL showed good adaptation to the lung tissue. Microscopically all animals presented good healing with deposition of fibrotic tissue layer on the GPBP and GPBPL. CONCLUSION: GPBP and GPBPL are an adequate materials to reinforce lung staple line, when resection of lung tissue was performed in dogs by thoracotomy or thoracoscopy.
Subject(s)
Animals , Male , Female , Cattle , Dogs , Pericardium , Bioprosthesis , Lung/surgery , Sutures , Thoracoscopy , Fibrosis , Thoracotomy , Tissue Adhesions , Glutaral , Thoracic Surgery, Video-Assisted , Wound Healing , Surgical Wound Dehiscence/prevention & control , Insufflation , Freeze Drying , Tissue Preservation , Airway Resistance , Suture TechniquesABSTRACT
Este trabajo de revisión incluye los principales aspectos anatómicos, histológicos, ultraestructurales, bioquímicos y fisiológicos del pulmón. El epitelio de las vías aéreas está formado por las siguientes células: ciliadas, caliciformes, serosas, en cepillo, intermedias, oncocitos, neuroendocrinas, de Kultschitzky y de Clara. Las más abundantes son las ciliadas, sus cilios poseen nueve pares de microtúbulos periféricos y dos centrales, y los brazos de dineína interno y extrerno y ATP-asa, responsables de su movilidad. Las segundas células más frecuentes son las caliciformes. Son las encargadas de la producción de moco. La célula de Clara se localiza en la vía aérea pequeña, posee gran actividad metabólica, con función destoxificadora de sustancias extrañas, y participa en la formación del surfactante. El alvéolo está compuesto por los neumocitos tipos I y II. El primero forma, junto con la célula endotelial, la membrana alvéolocapilar, que es la unidad anatómica-funcional del pulmón donde se lleva a cabo el intercambio gaseoso. El neumocito tipo II se encarga de la restitución del epitelio alveolar y es el principal productor del surfactante. El intersticio está formado por fibras, principalmente de colágena y elastina, por diversas sustancias como los proteoglicano y por células, siendo la más común el fibroblasto. Se mencionan también las células de la inflamación y la respuesta inmunitaria